Protein Purification
Sigma Aldrich Fine Chemicals Biosciences C myc proto oncogene human
MYCnbsponcogene is a global transcription factor. The gene is located on human chromosome 8q24.21. MYCnbsponcogene is a cancer promoting gene.
IBA LifeSciences Strep-TactinXT 4Flow HC
Strep-TactinXT 4Flow high capacity is perfectly suited for purifying Strep-tagII or Twin-Strep-tag fusion proteins. This resin offers excellent pressure stability, pH compatibility, and target purity (> 95%). Additionally, this resin is compatible with a broad range of reagents.Its high binding affinity makes it ideal for low abundant, high-expressing and challenging proteins. A low concentration of agarose also facilitates the purification of large proteins (>90 kDa). When working with, for example, multimeric proteins, choosing a resin with the Strep-Tactin ligand is recommended.Strep-TactinXT 4Flow high capacity resin has a higher density of immobilized Strep-TactinXT than the classic Strep-TactinXT 4Flowand thus can bind more protein.As the resin is optimized for column affinity chromatography, rather than batch purification, the suspension can be used to pack gravity flow columns.
Medchemexpress LLC C-Myc inhibitor 6 | 2768765-58-6 | 98.0% | 424.30 | 5 MG
c-Myc inhibitor 6 (compound A102) is a c-Myc inhibitor. It decreases cancer cell viability and degrades c-Myc protein. It can be used for the research of c-Myc imbalance, such as cancer, cardiovascular diseases, and viral infection.
- Inhibits cell viability of HL-60 with an IC50 value less than 100 nM
- Dose-dependently degrades c-Myc protein in HL-60 cells
ABclonal Technology DDDDK-Tag Rabbit mAb
The FLAG-tag (DYKDDDDK) is a short polypeptide added to proteins via recombinant DNA technology. It is widely used for protein purification by affinity chromatography and for studying proteins in living cells. The tag helps isolate recombinant proteins from host proteins and enables purification of multi-subunit complexes without disrupting them. It is also used to obtain proteins suitable for x-ray crystallography. The FLAG-tag can be detected with specific antibodies, making it useful in assays like immunofluorescence, SDS-PAGE, and Western blotting, especially when no antibody exists for the target protein.
ABclonal Technology N-Myc/MYCN Rabbit pAb
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
IBA LifeSciences Strep-Tactin; 5 mg
Strep-Tactin is a streptavidin variant, which can be utilized for coating of different surfaces. It is optimized for the binding of Strep-tagII and Twin-Strep-tag fusion proteins but still maintains the ability to capture biotinylated targets. Therefore, it can be used for assay development and protein interaction studies, including experiments such as proximity-dependent biotin identification (BioID).
FUJIFILM BIOSCIENCES INC SuperSep(TM) Ace Mini, 10-20%, 17well, 10ea per pack
SuperSep Ace Mini is polyacrylamide gel for protein electrophoresis. This is even smaller than commom minigel. Using this can finish electrophoresis in 20 min.
Medchemexpress LLC CYGB Rat His-Myc 10ug
Recombinant rat cytoglobin (CYGB) fused to His and Myc affinity tags, supplied as a purified protein for research applications. This preparation is intended for biochemical assays, binding studies, and antibody validation; it is provided in microgram-scale quantities and shipped on cold packs to preserve integrity. Research use only.
- Purified recombinant protein suitable for biochemical assays and antibody validation.
- N-terminal His tag and C-terminal Myc tag for purification and detection.
- Approximate molecular weight 31 kDa enabling expected migration on SDS-PAGE.
- Purity around 90% as reported by the supplier.
- Supplied in microgram-scale amount and shipped on cold packs.
- For research use only.
ABclonal Technology PE Rabbit anti-DDDDK-Tag mAb
The FLAG-tag (DYKDDDDK) is a short polypeptide used in recombinant DNA technology to study and purify proteins. It enables protein purification via affinity chromatography, allowing separation of recombinant proteins from wild-type proteins. Its mild conditions preserve protein complexes, aiding structural studies like X-ray crystallography. FLAG-tagged proteins can be detected in assays using anti-FLAG antibodies, especially useful when no specific antibody exists for the target protein. Applications include immunofluorescence for cellular localization and detection by SDS-PAGE and Western blotting.
ABclonal Technology ABflo 647 Rabbit anti-Myc-Tag mAb
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
IBA LifeSciences StrepMAB-Immo conjugate DY-649; 50 µg
StrepMAB-Immo fluorescenct conjugates are murine, high-affinity monoclonal antibodies, which are especially suited for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like FACS, immunohistochemistry, and immunocytochemistry. The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tagII providing an N-terminal extension by a SerAla linker. However, the Twin-Strep-tag always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). StrepMAB-Immo is available as conjugate with different fluorescent dyes (DY-488, DY-549, DY-649). The absorption/emission maxima of the fluorescent dyes are: • DY-488: 493/518 nm • DY-549: 550-560/575 nm • DY-649: 655/676 nmPlease note: For detection of Strep-tagII or Twin-Strep-tag fusion proteins in western blots, StrepMAB-Classic or Strep-Tactin are recommended.
Medchemexpress LLC MUSK Mouse Myc Hi 50ug
Recombinant fragment of mouse Muscle-Specific Kinase (MuSK) with Myc and His-SUMO tags supplied as a lyophilized powder. The protein is expressed in E. coli and formulated for stability, with low endotoxin and manufacturer guidance for reconstitution, storage, and handling available in the product datasheet.
- Recombinant fragment covering amino acids E22-S494.
- Myc and His-SUMO affinity tags for detection and purification.
- Expressed in E. coli for high yield production.
- Low endotoxin level (<1 EU/μg) suitable for cell-based assays.
- Lyophilized formulation for extended shelf life and easier shipping.
- Stable at -20°C for up to two years from receipt when stored properly.
ABclonal Technology DDDDK-Tag Rabbit mAb
The FLAG-tag (DYKDDDDK) is a short polypeptide added to proteins via recombinant DNA technology. It is widely used for protein purification by affinity chromatography and for studying proteins in living cells. The tag helps isolate recombinant proteins from host proteins and enables purification of multi-subunit complexes without disrupting them. It is also used to obtain proteins suitable for x-ray crystallography. The FLAG-tag can be detected with specific antibodies, making it useful in assays like immunofluorescence, SDS-PAGE, and Western blotting, especially when no antibody exists for the target protein.